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巨噬细胞极化是免疫反应中的一个重要过程,它涉及巨噬细胞向不同功能状态的转变。其中,M1型巨噬细胞主要参与炎症反应和抗感染,而M2型巨噬细胞则在组织修复和免疫调节中发挥关键作用。在流式细胞术检测中,我们 巨噬细胞功能极化对机体抗病原菌感染及损伤修复的质量和效率具有重要作用。 巨噬细胞有不同的极化方式,如机体遇到细胞内病原微生物感染时,巨噬细胞会表现出经典型极化(M1)并表达相应的促炎因子TNF-α、IL-12及iNOS来清除病原微生物。 巨噬细胞的重要性:巨噬细胞是分布于全身的固有免疫细胞,在器官发育、组织稳态和免疫监视中发挥关键作用。 它们具有高度可塑性,能根据环境信号极化为不同表型,其中 M1(促炎)和 M2(抗炎) 是两种主要类型。
复旦彭勃团队揭示小胶质细胞碎片的清除机制 文献解读:高胆固醇饮食与脂肪肝相关肝癌的关系 数字病理的未来:透明化3D病理技术的崛起和应用展望 丁香通:为生物医药企业提供专业品牌运营服务 新品|Olink Reveal 基于 NGS 的新一代蛋白组学 不用荧光! 由于巨噬细胞在不同的组织环境和生理、病理条件下的异质性,巨噬细胞被分为两种主要类型:M1型巨噬细胞和M2型巨噬细胞 [1]。 研究发现,NR能够增加人类M1型巨噬细胞表面的一种名为CCR7的受体表达。 CCR7是一种化学趋化因子受体,对于指导巨噬细胞迁移至炎症或损伤部位至关重要。
巨噬细胞标记物 巨噬细胞的标记物包括CD68,CD80,CD163等等,当巨噬细胞出现极化时,M2亚型的细胞的标记物就变成了CD163,所以,可以通过免疫荧光双染的方法或者流式来确定M2亚型细胞的表达。
使用LPS和IFN-γ诱导RAW264.7细胞是建立M1型巨噬细胞模型的经典方法。流式细胞术是检测细胞表型和功能的强大工具。针对M1型巨噬细胞,我们可以从以下几个层面的指标进行染色和检测: 一、核心推荐指标(最常用、最特异) 这些指标是M1型巨噬细胞的经典标志物,检测它们最能直接证明极化成功. 巨噬细胞是三种吞噬细胞类型之一,还有粒细胞(中性粒细胞、嗜酸性粒细胞和嗜碱性粒细胞)和树突状细胞(DCs)。 一般来说,巨噬细胞可根据其功能和活化程度分为两个亚型:经典激活的M1巨噬细胞和交替激活的M2巨噬细胞;极化的这种性质表示频谱的相对. THP-1是一种人类单核细胞系,可以极化为不同的亚型,包括M1和M2巨噬细胞。 M1和M2巨噬细胞具有不同的功能和表型,M2巨噬细胞在抗炎反应和修复过程中起重要作用。
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